Contact an Account ManagerDevelopment and validation of an HPLC method for the simultaneous determination of tocopherols, tocotrienols and carotenoids in cereals after solid-phase extraction
Summary
Type: HPLC
Application #: 125550
Detection:
Conditions: Eluent A: methanol
Eluent B: methanol – isopropanol – acetonitrile (40:50:10, v/v/v)
Eluent C: methanol – isopropanol – acetonitrile (25:65:10, v/v/v)
Gradient: 100 % A linear to 100 % B in 10 min (flow rate: 1 to 1.2 mL/min in 10 min), to 100 % C in 5 min (flow rate: 1.2 mL/min constant), 100 % C for 1 min (flow rate: 1.2 mL/min constant)
Temperature: 25 °C
Authors: Irakli, M. N. et al.
Source: J. Sep. Sci. 2011, 34, 1375–1382
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